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Home My WebLink AboutCORRESPONDENCE - PURCHASE ORDER - 9122983Proposal Transmittal Information Date: May 7, 2012 To: John Stephen, CPPO, CPPB Senior Buyer City of Fort Collins JStephen@fcgov.com CO'O Ta e ,11, sity Sponsored Programs Fort Collins, CO 80523-2002 Phone: (970) 491-6355 Fax: (970)491-6147 � t �z ?93 Mike Calhoon City of Fort Collins — Parks Department Parks Supervisor 413 S. Bryan Ave. Fort Collins, CO 80521 MCalhoon@fcgov.com Colorado State University submits an original proposal entitled "Testing of Mosquito Pools for West Nile Virus, City of Fort Collins, 2012." Dr. Chester Moore is CSU's Principal [nvestigator. The Research Administrators assigned to this project are: Linda Monum Tracey Castaneda Senior Research Administrator Research Administrator Sponsored Programs, CSU Sponsored Programs, CSU Fort Collins CO 80523-2002 Fort Collins CO 80523-2002 Phone (970) 491-0974 Fax (970) 491-6147 I inda. monumAco lostate. edu Phone: (970) 491-1560 Fax: (970) 491-6147 tracey.castaneda(c ,,co lostate.edu Please direct questions, correspondence or award documents to the above individual. Thank you. Enc: proposal Project Proposal (PASS #113864) Testing of Mosquito Pools for West Nile Virus, City of -Fort Collins, 2012 BACKGROUND History of WNV in Colorado and the Front Range: West Nile virus (WNV) first appeared in Colorado in the late summer of 2002. Since that time Colorado, and particularly the northern Front Range counties (Larimer, Weld, Boulder), have seen WNV cases in humans in every year. Case data for the City of Fort Collins are not available, but it is reasonable to assume that a significant proportion of Larimer County cases occurred in Fort Collins residents. In Larimer County, the annual number of cases reported has ranged from a high of 546 in 2003, to a low of a single case in 2011, for a total of 767 cases (Table 1). This represents about 17% of the total cases reported in Colorado, even though Larimer County makes up only about 6% of the state's population. Data from the past ten years suggest that Latimer County, and the northern Front Range in general, are at higher risk of West Nile virus transmission than other parts of the state. The City of Fort Collins responded to the WNV emergency in 2003 by establishing a surveillance and control program which, in all likelihood, has reduced the level of WNV transmission within the City limits. The City uses an independent contractor to carry out the program. Guidance and supervision is provided by City staff. The surveillance component of the program involves extensive monitoring of potential larval mosquito habitats as well as weekly collection and testing of adult Culex mosquitoes for the presence of WNV. The primary components of the control portion of the program focus on source reduction, larviciding, and public education. Adulticiding is reserved as an option to be used under exceptional levels of virus activity within the community. Information obtained from the adult trapping and testing program is essential to deciding if, where, and when adulticiding may be necessary. Table L Reported West Nile virus infections in humans, 2003-2011, Larimer County and statewide. Data from Colorado Dept. of Health and Environment (available on the Internet at: httt)://www.edi)he.state.co.us/dc/zoonosis/wnv/index.html). Year County cases Fatalities State cases % of state cases 2002 0 0 14 0.0 2003 546 9 2947 18.5 2004 17 0 291 5.8 2005 13 0 106 12.3 2006 42 1 345 12.2 2007 97 0 578 16.8 2008 13 1 71 18.3 2009 25 0 102 24.5 2010 13 0 81 16.0 2011 1 0 7 14.3 Total 767 11 4542 16.9 From 2003 through 2003 the U.S. CDC's Division of Vector -Borne Infectious Diseases provided virus testing of mosquito pools at no charge. With changing priorities and funding, that service was no longer available beginning with the 2009 season. Colorado State University's (CSU) Arthropod-bome & Infectious Disease Laboratory (AIDL) had been conducting parallel studies of WNV along the Front Range, including virus testing. Thus, we are ideally suited to provide the testing service previously offered by the CDC at a competitive cost. Resources available at CSU include highly trained staff and students, laboratory space in the Infectious Diseases Annex (IDA), equipment and reagents to perform polymerise chain reaction (PCR) detection of WNV, and standard laboratory equipment such as microscopes, freezers, incubators, etc. PROJECT PROPOSAL A. Period of contract 1. The period of this contract shall extend from June 1 to September 15, 2012 inclusive B. Scope of work 1. Work plan a. Mosquito traps are placed and retrieved weekly at approximately 41 sites by City's contractor. Site locations, frequency of trap placement, and related issues to be determined by City in coordination with contractor. b. Mosquitoes are identified by contractor staff and Cidex females (Codex tarsalis and Cidex spp.) are placed in pools of up to 100 individuals, separated by collection site and collection date. CSU will provide the City's contractor with standard vials to store and transport the mosquito pools (this eliminates the time- consuming task of transferring specimens once they arrive at IDA). c. Contractor delivers the weekly collection of pools to IDA according to an agreed upon schedule (typically early Friday afternoon) d. IDA staff receives specimens from contractor and places in appropriate storage/refrigeration to await processing e. IDA staff conducts spot checks to identify mis-labeling, missing pools, etc., then processes pools and performs PCR testing f. IDA staff assembles and interprets PCR results and reports to City of Fort Collins and other organizations as needed. 2. Deliverables a. Up to 1000 pools will be tested under the contract. b. Test results will be provided to City within 60 hr (2.5 working days) of beginning the testing process. i. Specimens must be received by Friday at 1:00 (1300 hr) for results to be available the following week ii. Pools will be processed in order of collection date (earliest collections processed first) unless otherwise requested by the City's coordinator iii. To obtain more rapid test results (e.g., when a sudden increase in virus activity is suspected) pools can be accepted early in the week for expedited testing. iv. Example reports are shown in the Appendix Staffing a. PI (C.G. Moore) and Co-PI's (G. Ebel, L. Eisen) will oversee day-to-day operation of the project, check for labeling errors, etc.; collate and interpret laboratory data; and transmit reports to City of Fort Collins. b. Lab Technician will receive mosquito pools from contractor, prepare specimens accordinj to protocol;'perform PCR testing; maintain all laboratory notebooks documenting dates and times, pool numbers, results, and other relevant information; prepare summaries of tests; and deliver to PI in a timely fashion. Position is Student Lab Asst. I, Step 2 ($9.50/hr). C. Budget The collection and testing components of the project will take place from June 1, 2012 to August 31, 2012. Funding will continue until September 15, 2012 to permit data analysis and preparation of the final project report. The attached budget (Tables 2 and 3) costs to process and test mosquito specimens for West Nile virus antigen via PCR testing. The budget covers West Nile virus testing only. It does not include testing for other potential pathogens of human or veterinary importance. Table 2. Proposed budget for Fort Collins WNV testing, 2012 season. Budget Item Cost PERSONNEL SALARIES Academic Faculty: $7,194 Fringe Rate $165 Student Hourly: $1,482 Fringe Rate $15 TOTAL SALARY: $8,676 TOTAL FRINGE: $180 TOTAL PERSONNEL: $8,856 DOMESTIC TRAVEL: $0 MATERIALS AND SUPPLIES $8,205 OTHER DIRECT COSTS Equipment Use Fees: $0 Animal Care: $0 TOTAL OTHER DIRECT: $0 EQUIPMENT: $0 TOTAL DIRECT COSTS: $17,061 Facilities & Administrative: $5,340 Consortium F&A $0 TOTAL: $22,401 Table 3. Materials and supplies for WNV testing. Item Description Cost/1o0o pools 100bp ladder, 32uL $ 203.00 8-strip PCR tubes 0.2 ml), 26 ea. $ 339.00 A arose, 6 $ 96.00 Centrifuge tubes 1.5 ml), 202 ea $ 188.00 10mM dNTP solution, 300 uL $ 110.00 Falcon tube 5ml , 100 ea $ 334.00 Forward primer, 100uL $ 6.00 Loading dye, 200uL $ 51.00 Minimum Essential Medium, 175 ml $ 88.00 Nuclease -free water, 7.92 ml $ 15.00 Pipette tips 20, 200, 2000uL , 702 ea $ 961.00 QlAmp Viral RNA Mini Kit, 100 ea $4,048.00 Re eator Pi ettor tips, 7 ea $ 116.00 Reverse primer. t00uL $ 12.00 Reverse transcri tase/Buffer/0.1 M DTT $ 567.00 Storage box, 2 ea. $ 219.00 Tag of merase/Buffer, 100 $ 576.00 2.5mM dNTP solution, 300 uL $ 110.00 Software renewal/update, ArcGIS $ 50.00 Software renewal/update, EndNote $ 116.00 $8,205.00 APPENDIX A. Report of all positive pools by trap location, collection date, trap type, and species collected ity: .Week' M ool 2009 West Nile -positive Pools, Ft. Collins Loveland I A ool te' - -City Week` CMC Pool IDA'Pool - Date- 'Site '- Desrnphon - - --Method U0281 10198 747ur-08 CV'060— Nam eLi cohareco slate urJr«nm eePo of Micro., Nmunal.. Partial. awapoa-0wne infectious oiseasee laboratory Fat Caere, c060693-16M 97Ua9fmm l�ig�it— Spec�u PoolSizei Gvse6s —9C VY009-13:0I PM 'Sne - _.. ?DesrnPhon u- I. u un- a au re rrer nve a otte hat gn pipiens I FG 2 GSU-0316 10233 15Ju1-09 FC-031 Wlllav Spnngs hl L9rsali5 10fi PC 29 0373 CSU-PC 10290 1]Jul-09 FG029 Bens Park Gravid ipkns 115 29 - 1 1 1JJul C-034 Country Club Lght piptens 1 PC 3 CSU-0368 10305 21-Jul-09 FC-036 Hembck Light tarsalis I W PC 30 CSU-0394 10311 21Ju1-Jul FG-019 Etlwa Palk Light tarsalis 67 PC 30 CSU-W 19 10336 22Ju1-09 FG039 Fossil Creek Sauth (Greenstone) Light Iarsalis 86 PC 30 CSU-0427 10344 22Ju1-09 FGOb1 Well Chase Kerhter Farm Light tarsalis 21 PC 30 CSU-0439 10356 22Ju1-Jul FC-027 San Luis Light tarsalis 99 PC 30 CSU-0415 10362 22-Jul-09 FGOa6 725 Weslshore Court Light twsalis at PC 30 CSU-0451 1W68 23-Jul-09 FGi]61 Holley Environ. Rant Research CV Light Nrsalis IW PC 30 CSU-0456 10373 23-Jul-09 FC-IN1 Fishback Li hl lalsalis 100 -u an ment uurt gl br5a i5 PC 31 CSU-0553 10170 27-Jul-09 FC-038 Lockside Lane Light hrsalis 31 FG 31 GSU-0561 10479 2]-Jul-09 FC-036 Hemk,ck U hI larsalis 91 PC 31 GSU-0576 10493 27-Jul-09 FC-066 Prospect Pords Drake Water Gravid pipiens la FG 31 CSU-0577 10494 2]-Jul-09 FGO66 Prospect Po1Ms Drake Water Light tarsalis 69 PC 31 CSU-0604 10521 28Ju609 FC-017 KeenlaM S Twin Oak li hl i lens 2 FG 31 CSU-0608 10525 29-Jul-09 FGOfiI H.11ey Errvimm Plant Research Ctr Li hl i ens O 1 U 1 I Jul FG041 Fshback ght tarsalis 4 B. Weekly summary report shows summary data for each species, along with indices and graphical display of vector and virus abundance. 200 West Nile Virus Testing. Weekly Summary R. Cdlirs. C..'Vens r° rd� a <a, xinnvxrvn�ple week vvvx xv �+n Fc Colllnr. C+. can;Os xa. 3 �8� n nnvnnvnnv .nssyssxx.x Sample wnk ° C. Display of Cidez abundance for the current season against historical abundance data. Pon Collins L—Il xuro,iul Culex oa; zO 2M a. cmmt rem Mn) Oda na 5" m f[ CWfns C., tars'., AMM ,u 5 is _is .—a, I.a.. Fc Cdlim cr. yµens.wa os Loveland Ca. r u,a.wm •, suw.. w�lci vrs'S'I+b m.lc:ow.ulsd<+[�Ine,.m�+ ass en I I ass 5„ pipiens, 2000-08 i n z.m m m m m ss: w�` ,. ,1 m m m m :' m m m mwµm ., n m >. ss. r< m m >, m m